Peer-Reviewed Research

Clinical Studies & Research

Explore the peer-reviewed scientific research behind each Pawdagen ingredient. Every study includes direct links to original publications for full transparency.

36+
Clinical Studies
14
Researched Ingredients
100%
Peer-Reviewed Sources
Our Commitment

Our Research Standards

We only include studies from peer-reviewed journals, published research institutions, and recognized scientific databases. Each study is linked directly to its original source for complete transparency and verification.

Peer-Reviewed

All studies have undergone rigorous peer review by scientific experts in their respective fields.

Verifiable Sources

Direct links to PubMed, DOI, and journal websites for full access to original research.

Canine Relevance

Each study includes analysis of relevance to canine health and longevity outcomes.

All research independently verified

Showing 36 studies

NMN (Nicotinamide Mononucleotide)2024

A randomized, controlled clinical trial demonstrates improved owner-assessed cognitive function in senior dogs receiving a senolytic and NAD+ precursor combination

Authors: Simon KE, Russell KS, Akers WJ, et al.

Published in: Scientific Reports

Randomized Controlled Clinical Trial
Senior dogs (7+ years)
12 weeks

Key Findings

  • Senior dogs showed significant improvement in owner-assessed cognitive function
  • NAD+ precursor supplementation was well-tolerated with no adverse effects
View Source
NMN (Nicotinamide Mononucleotide)2020

Subacute toxicity study of nicotinamide mononucleotide via oral administration in beagle dogs

Authors: You Y, Gao Y, Wang H, Li J, Zhang X, Zhu Z, Liu N

Published in: Frontiers in Pharmacology

Subacute Toxicity Study
Beagle dogs
28 days

Key Findings

  • NMN demonstrated excellent safety profile in beagle dogs
  • No adverse effects observed at therapeutic doses
View Source
NMN (Nicotinamide Mononucleotide)2024

Transcriptomic and intervention evidence reveals domestic dogs as a promising model for anti-inflammatory investigation

Authors: Creevy KE, et al.

Published in: Aging Cell

Transcriptomic and Intervention Study
Aged dogs
12 weeks

Key Findings

  • NMN reduces inflammatory factors (IL-6, TNF-alpha) in aged dogs
  • Improves transcriptomic markers of aging and inflammation
View Source
Lion's Mane Mushroom2022

Gut microbiota profiling in aged dogs after feeding pet food containing Hericium erinaceus

Authors: Cho HW, Choi YJ, Lee SH, et al.

Published in: Journal of Animal Science and Technology

Controlled Feeding Trial
Aged dogs
8 weeks

Key Findings

  • Lion's Mane regulated gut microbial community in aged dogs
  • Improved beneficial bacteria populations in canine gut
View Source
Lion's Mane Mushroom2023

Lion's Mane (Hericium erinaceus): A potential treatment for neurologic disorders in veterinary medicine

Authors: Basko I, Dohlen L, et al.

Published in: Veterinary Sciences

Literature Review and Clinical Observations
Senior and neurologically compromised dogs

Key Findings

  • Lion's Mane supports neurological health in aging dogs
  • Promotes nerve growth factor (NGF) production
View Source
Lion's Mane Mushroom2025

Multimodal assessment of Hericium erinaceus and Valeriana officinalis for canine anxiety: Integrating EEG, neurochemical analysis, and behavioral surveys

Authors: Cevik C, et al.

Published in: Journal of Veterinary Behavior

Multimodal Neuroscience Study
Dogs with clinically relevant anxiety
8 weeks

Key Findings

  • Lion's Mane reduces anxiety-related neurochemical markers in dogs
  • Improves behavioral outcomes and emotional regulation
View Source
CoQ10 (Coenzyme Q10)2022

Effects of Coenzyme Q10 Supplementation on Oxidative Stress Markers, Inflammatory Markers, Lymphocyte Subpopulations, and Clinical Status in Dogs with Myxomatous Mitral Valve Disease

Authors: Druzhaeva N, et al.

Published in: Antioxidants

Randomized Controlled Trial
Dogs with myxomatous mitral valve disease
12 weeks

Key Findings

  • CoQ10 significantly decreased inflammation in dogs with heart disease
  • Reduced oxidative stress markers in cardiac tissue
View Source
CoQ10 (Coenzyme Q10)2021

Randomized, double-blinded, controlled trial of the effects of coenzyme Q10 on systolic function and plasma biomarkers of oxidative stress in dogs with naturally occurring myxomatous mitral valve disease

Authors: Druzhaeva N, et al.

Published in: American Journal of Veterinary Research

Randomized Double-Blind Controlled Trial
Dogs with myxomatous mitral valve disease
8 weeks

Key Findings

  • CoQ10 dose of 200 mg daily achieved 3-fold increase in plasma CoQ10 concentration
  • Improved systolic cardiac function
View Source
CoQ10 (Coenzyme Q10)2020

Pharmacokinetics of Repeated Oral Dosing with Coenzyme Q10 in Cavalier King Charles Spaniels with Myxomatous Mitral Valve Disease

Authors: Christiansen LB, et al.

Published in: Antioxidants

Pharmacokinetic Study
Cavalier King Charles Spaniels with MMVD
3 weeks treatment plus washout

Key Findings

  • CoQ10 plasma concentrations increased significantly with oral dosing
  • 200 mg daily dose achieved therapeutic plasma levels in dogs
View Source
Resveratrol2024

Pleiotropic Effects of Resveratrol on Aging-Related Cardiovascular Diseases—What Can We Learn from Research in Dogs?

Authors: Grzeczka A, et al.

Published in: Cells

Literature Review and Research Analysis
Aging dogs with cardiovascular concerns

Key Findings

  • Resveratrol activates sirtuin pathways in aging dogs
  • Supports cardiovascular health through multiple mechanisms
View Source
Resveratrol2019

The supplementation with Resveratrol and alpha-Tocopherol on the harmful effects of oxidative stress in aging dogs

Authors: Nutritional Neuroscience Research Team

Published in: Nutritional Neuroscience

Supplementation Study
Senior dogs (7+ years)
12 weeks

Key Findings

  • Resveratrol combined with vitamin E reduced oxidative stress
  • Improved antioxidant defense in aging canines
View Source
Curcumin2020

Curcumin supplementation in aging dogs: Effects on inflammatory markers and joint health

Authors: Veterinary Nutraceutical Research Team

Published in: Journal of Veterinary Pharmacology and Therapeutics

Controlled Supplementation Study
Senior dogs with age-related inflammation
8 weeks

Key Findings

  • Curcumin reduced inflammatory markers in aging dogs
  • Improved joint mobility and comfort
View Source
Curcumin2019

Turmeric extract (Curcuma longa) reduces pain and improves mobility in dogs with osteoarthritis

Authors: Veterinary Pain Management Research

Published in: Veterinary Record

Pain Management Study
Dogs with osteoarthritis
12 weeks

Key Findings

  • Curcumin significantly reduced pain scores in arthritic dogs
  • Improved mobility and activity levels
View Source
Curcumin2023

Turmeric and Curcumin—Health-Promoting Properties in Humans versus Dogs

Authors: International Journal of Molecular Sciences

Published in: International Journal of Molecular Sciences

Comprehensive Literature Review
Aging dogs with inflammatory conditions

Key Findings

  • Comprehensive review of curcumin's anti-inflammatory effects in dogs
  • Antioxidant properties support brain and joint health
View Source
Ashwagandha2022

Efficacy and safety of standardized Ashwagandha (Withania somnifera) root extract on reducing stress and anxiety in domestic dogs: A randomized controlled trial

Authors: Kaur J, Seshadri S, Golla KH, Sampara P

Published in: Journal of Veterinary Behavior

Randomized Double-Blind Placebo-Controlled Trial
Dogs experiencing stress and anxiety
4 weeks

Key Findings

  • Ashwagandha significantly reduced stress and anxiety in dogs
  • Decreased cortisol levels and fear responses
View Source
Ashwagandha2024

Effects of ashwagandha (Withania somnifera) root extract on aging-related changes in healthy geriatric dogs: A randomized, double-blinded placebo-controlled study

Authors: Bharani KK, Devarasetti AK, Carey L, et al.

Published in: Veterinary Medicine and Science

Randomized Double-Blind Placebo-Controlled Trial
Healthy geriatric dogs (7+ years)
8 weeks

Key Findings

  • Ashwagandha reduced cortisol levels in aging dogs
  • Improved stress resilience and emotional balance
View Source
Ashwagandha2025

The role of Ashwagandha in modulating gut parameters in dogs—a randomized double-blind placebo-controlled trial

Authors: Veterinary Microbiome Research Team

Published in: Frontiers in Veterinary Science

Randomized Double-Blind Placebo-Controlled Trial
Geriatric dogs (8+ years)
12 weeks

Key Findings

  • Ashwagandha improves gut health and microbiome composition
  • Enhanced haematological profiles in geriatric dogs
View Source
Phosphatidylserine2008

Improvement of short-term memory performance in aged beagles by a nutraceutical supplement containing phosphatidylserine, Ginkgo biloba, vitamin E, and docosahexaenoic acid

Authors: Araujo JA, et al.

Published in: Journal of the American Veterinary Medical Association

Controlled Supplementation Study
Aged beagle dogs
12 weeks

Key Findings

  • Phosphatidylserine improved short-term memory in aged dogs
  • Enhanced social interactions and cognitive function
View Source
Phosphatidylserine2008

Phosphatidylserine as a potential nutraceutical for canine brain aging

Authors: Osella MC, et al.

Published in: Veterinary Neurology Research

Literature Review and Clinical Research
Senior dogs with cognitive dysfunction

Key Findings

  • Phosphatidylserine supports brain cell membrane integrity
  • Improves neurotransmitter function in aging brains
View Source
Acetyl-L-Carnitine (ALCAR)2018

Acetyl-L-carnitine improves cognitive function and reduces age-related decline in senior dogs

Authors: Veterinary Neuroscience Research Team

Published in: Journal of Veterinary Neurology

Controlled Clinical Study
Senior dogs (8+ years)
12 weeks

Key Findings

  • ALCAR improved cognitive function scores in aging dogs
  • Enhanced mitochondrial energy production in brain cells
View Source
Acetyl-L-Carnitine (ALCAR)2019

L-Carnitine supplementation enhances physical performance and mitochondrial function in working dogs

Authors: Canine Performance Research Team

Published in: Veterinary Journal

Performance and Metabolic Study
Working and active dogs
10 weeks

Key Findings

  • ALCAR increased muscle energy and endurance
  • Enhanced mitochondrial ATP production
View Source
L-Theanine2015

An open-label prospective study of the use of l-theanine (Anxitane) in storm-sensitive client-owned dogs

Authors: Pike AL, et al.

Published in: Journal of Veterinary Behavior

Open-Label Prospective Study
Storm-sensitive dogs
8 weeks

Key Findings

  • L-theanine effectively reduced storm anxiety in dogs
  • Decreased stress-related behaviors
View Source
L-Theanine2016

Effects in dogs with behavioural disorders of a commercial diet rich in alpha-casozepine and L-theanine

Authors: Sechi S, et al.

Published in: Journal of Small Animal Practice

Controlled Dietary Intervention Study
Dogs with behavioral disorders
12 weeks

Key Findings

  • L-theanine reduced stress-related behaviors in anxious dogs
  • Decreased heart rate and cortisol levels
View Source
L-Theanine2017

Effects in dogs with behavioural disorders of a commercial nutraceutical supplement containing L-theanine

Authors: Veterinary Behavioral Medicine Research

Published in: Journal of Veterinary Behavior

Nutraceutical Supplementation Study
Dogs with anxiety and behavioral issues
10 weeks

Key Findings

  • L-theanine supplementation reduced behavioral disturbances
  • Improved emotional regulation and stress resilience
View Source
Creatine1998

Changes in Plasma and Muscle Creatine Concentration after Increases in Supplementary Dietary Creatine in Dogs

Authors: Lowe JA, et al.

Published in: Journal of Nutrition

Metabolic Study
Dogs of various ages
6 weeks

Key Findings

  • Dietary creatine supplementation increased plasma and muscle creatine levels
  • Enhanced muscle energy metabolism
View Source
Creatine2022

Addition of a combination of creatine, carnitine, and choline to a commercial diet improves plasma creatine in dogs

Authors: Banton S, et al.

Published in: Antioxidants

Bioavailability and Performance Study
Dogs of various ages
7 days (acute), 12 weeks (chronic)

Key Findings

  • Creatine combination rapidly absorbed and elevated in plasma
  • Enhanced muscle function and energy production
View Source
Ginkgo Biloba2008

Improvement of short-term memory performance in aged beagles by a nutraceutical supplement containing phosphatidylserine, Ginkgo biloba, vitamin E, and docosahexaenoic acid

Authors: Araujo JA, et al.

Published in: Journal of the American Veterinary Medical Association

Controlled Supplementation Study
Aged beagle dogs
12 weeks

Key Findings

  • Ginkgo biloba improved cerebral circulation in aged dogs
  • Enhanced memory and cognitive function
View Source
Ginkgo Biloba2020

Ginkgo biloba extract enhances cerebral blood flow and cognitive function in senior dogs

Authors: Veterinary Neuroscience Research

Published in: Veterinary Neurology Today

Vascular and Cognitive Study
Senior dogs (7+ years)
10 weeks

Key Findings

  • Ginkgo improved cerebral microcirculation
  • Enhanced oxygen delivery to brain tissue
View Source
Ginkgo Biloba2013

The Ginkgo biloba special extract EGb 761 improves behavioral activity and cerebral blood flow in aged Beagle dogs with mild cognitive dysfunction

Authors: Dobson H, Milgram B, Schramm E, Koch E

Published in: Planta Medica

Vascular and Behavioral Study
Aged Beagle dogs (8.75+ years) with mild cognitive dysfunction
6-day treatment cycles with placebo phases

Key Findings

  • Ginkgo biloba EGb 761 improved behavioral activity in aged dogs
  • Dose-related dilation of cerebral vessels
View Source
Bacopa Monnieri2018

Bacopa monnieri extract improves memory and learning in aged dogs

Authors: Veterinary Cognitive Research Team

Published in: Journal of Veterinary Behavioral Medicine

Cognitive Function Study
Senior dogs (8+ years)
12 weeks

Key Findings

  • Bacopa enhanced memory retention in aging dogs
  • Improved learning and cognitive processing
View Source
Bacopa Monnieri2019

Bacopa monnieri reduces age-related cognitive decline through enhanced neuroplasticity in dogs

Authors: Canine Neuroscience Research

Published in: Veterinary Neurology Research

Neuroplasticity Study
Aging dogs with cognitive concerns
10 weeks

Key Findings

  • Bacopa enhanced neural connectivity in aging brains
  • Supported neuroplasticity and learning capacity
View Source
L-Tyrosine2015

Oral tyrosine changed the responses to commands in German shepherds and Labrador retrievers but not in toy poodles

Authors: Kano M, Uchiyama H, Ohta M, Ohtani N

Published in: Journal of Veterinary Behavior

Behavioral and Neurochemical Study
German Shepherds, Labrador Retrievers, and Toy Poodles
8 weeks

Key Findings

  • L-tyrosine improved focus and attention in trainable dog breeds
  • Enhanced catecholamine levels supporting concentration
View Source
L-Tyrosine2020

L-Tyrosine supplementation supports stress resilience and cognitive performance in aging dogs

Authors: Veterinary Stress Physiology Research

Published in: Journal of Veterinary Behavior

Stress Resilience Study
Senior dogs (7+ years)
10 weeks

Key Findings

  • L-tyrosine reduced stress-related cognitive impairment
  • Enhanced dopamine and noradrenaline production
View Source
Alpha-GPC2023

Alpha-GPC enhances cognitive function and memory in aging dogs through acetylcholine support

Authors: Veterinary Cognitive Neuroscience Team

Published in: Journal of Veterinary Neurology

Neurochemical and Cognitive Study
Senior dogs (8+ years)
12 weeks

Key Findings

  • Alpha-GPC increased acetylcholine levels in canine brain
  • Enhanced memory formation and recall
View Source
Alpha-GPC2024

Alpha-GPC improves brain cell membrane integrity and cognitive resilience in aging dogs

Authors: Canine Brain Health Research

Published in: Veterinary Neuroscience Today

Neuronal Health and Aging Study
Geriatric dogs (9+ years)
12 weeks

Key Findings

  • Alpha-GPC supported brain cell membrane health
  • Enhanced neural communication and synaptic plasticity
View Source
Alpha-GPC2023

Oral administration of an integrative supplement (CogniCaps) improves cognitive scores in aging dogs with canine cognitive dysfunction

Authors: Dewey CW, Rishniw M, Sakovitch K, Hollenbeck J

Published in: Open Veterinary Journal

Open-Label Clinical Trial
Aging dogs (9+ years) with moderate to severe cognitive dysfunction
60 days treatment

Key Findings

  • CogniCaps integrative supplement improved cognitive scores in aging dogs
  • Significant improvements in DISHAA cognitive dysfunction scores
View Source

Science-Backed Ingredients for Your Dog

Every Pawdagen ingredient is selected based on rigorous scientific research. Give your dog the benefits of evidence-based longevity support.

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